The widespread commensal intestine bacterium Bacteroides thetaiotaomicron makes use of section separation of the transcription termination issue Rho to colonize and thrive within the mammalian intestine, in accordance with a brand new examine in mice.
The findings recommend that section separation may be important for different essential intestine microbes and related for novel microbiome-based medical functions. The intestine microbiota performs a essential position in human well being. Manipulating intestine commensal communities might present promising therapeutic pathways for treating a number of illnesses. Nonetheless, this aim requires understanding mechanisms that allow helpful micro organism to colonize the intestine – a posh course of that features profitable competitors for scarce vitamins and resistance to the host’s immune system. Right here, Emilia Krypotou and colleagues consider these mechanisms in B. thetaiotaomicron, one of the ample bacterial species within the human intestine of wholesome people and a species at present being examined in medical trials as a possible therapeutic for gastrointestinal issues. The authors targeted on the extremely conserved transcription termination issue Rho, which is crucial in regulating gene transcription in micro organism.
Nonetheless, not like different micro organism, B. thetaiotaomicron’s Rho protein harbors a big intrinsically disordered area (IDR). Krypotou et al. now present that the distinctive IDR of this Rho protein allows liquid-liquid section separation of the transcription termination issue and is essential for B. thetaiotaomicron gene regulation within the intestine. Via in vitro and in vivo experiments in a mouse mannequin, the authors discovered that B. thetaiotaomicron responded to the mammalian intestine atmosphere by sequestering Rho molecules inside a membraneless compartment through section separation. This IDR-dependent molecular condensation elevated Rho termination exercise, ensuing within the modified transcription of tons of of genes, together with a number of required for intestine health and colonization.
Krypotou, E., et al. (2023) Micro organism require section separation for health within the mammalian intestine. Science. doi.org/10.1126/science.abn7229.